MAINE
PUBLIC HEALTH ALERT NETWORK SYSTEM
Maine Department of Health
and Human Services
Maine Center for Disease
Control and Prevention (Maine CDC)
(Formerly Bureau of Health)
11 State House Station
Augusta, Maine 04333-0011
Phone 1-800-821-5821 / Fax 207-287-7443
2006PHADV010
TO: Infection Control
Practitioners, Emergency Departments, Primary Care Association, Maine Hospital
Association, Veterinarians, Maine HHS Public Health, Maine HHS Public Health
Leadership, State and Federal Agencies, Public Health Nurses
FROM: Dora
Anne Mills, M.D., M.P.H., Public Health Director
SUBJECT: Human
Arbovirus Update for Healthcare Providers in Maine, June 2006
DATE: June
13, 2006
TIME: 7:00 AM
PAGES: 3
PRIORITY: Review
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In 2005, two horses, one bird, and one mosquito pool tested positive for Eastern Equine Encephalitis (EEE) in Maine, all in York County. In the same year, eight birds tested positive for West Nile virus (WNV). These results are the latest indication that there is a real risk of EEE and WNV transmission from infected mosquitoes to Maine residents. As the warm season approaches, the Maine CDC is alerting physicians and other healthcare providers of the risk of acquired human EEE and WNV infections, and providing laboratory testing criteria and guidelines.
Clinical Features of
Arbovirus Infection
EEE: Symptoms of EEE usually appear 3 to 10 days after the bite of an infected mosquito, and range from mild flu-like illness to encephalitis, coma, and death. The EEE case fatality rate is about 35%-50%. It is estimated that 35% of people who survive EEE will have mild to severe neurological deficits.
WNV: The incubation period for WNV in humans
ranges from 2 to 15 days. However, most people infected with WNV do not show any
symptoms. Mild WNV infections can cause fever, headache and body aches, often
with a skin rash and swollen lymph glands. More severe infections can cause
headache, high fever, neck stiffness, stupor, disorientation, coma, tremors,
convulsions, paralysis and, sometimes, death.
Human cases of EEE occur
relatively infrequently, largely because the primary transmission cycle takes
place in swamp areas where human populations tend to be limited. WNV probably infects more people than is
known because about 80% of people infected remain asymptomatic. The following
groups of people are at the greatest risk for both EEE and WNV infection:
Ø
Residents
of and visitors to endemic areas
Ø
People
who engage in outdoor work and recreational activities
Ø Persons over age 50 (EEE and WNV)
and younger than age 15 (EEE)
Diagnostic
Tests for EEE and WNV Infections
Clinical Suspicion: EEE and WNV infections can be suspected based on clinical symptoms
and patient history. Diagnosis relies
on a high index of suspicion and on results of specific laboratory tests. EEE,
WNV or other arboviral infections should be seriously considered in any
individual – but especially those over age 50 or younger than age 15 - who has onset of unexplained encephalitis,
meningitis, or high fever in the late summer or early fall. The local presence of EEE and WNV enzootic
activity should further raise the index of suspicion.
Laboratory Tests: Laboratory testing is required
for a confirmed diagnosis. The most efficient diagnostic methods are listed
below:
Ø Detection of IgM antibody in
serum collected 3-10 days after onset of illness (note: if a specimen collected
less than 10 days after onset of illness is negative, a convalescent serum
should be collected and tested for IgM antibody 2-3 weeks after the first
collection date).
Ø Detection of IgM antibody in
cerebrospinal fluid collected 3 to 10 after onset of illness (for persons with
neuroinvasive disease).
Because some other arboviral infections can
cause indistinguishable clinical presentations, public health testing for EEE
and WNV infections in Maine is accompanied by tests for the viruses that cause
St. Louis Encephalitis and Powassan Encephalitis. Specimens that are positive
by IgM at the Maine Health and Environmental Testing Laboratory (HETL) are sent
to the federal CDC in Atlanta for confirmatory testing using the
plaque-reduction neutralization (PRNT) technique. PRNT is the current gold standard for ruling out possible false
positive results and in distinguishing cross-reactions that can occur between
different arboviral infections.
Diagnostic testing of serum and
cerebrospinal fluid for arbovirus infections is available free of charge
through HETL. To ensure a
quicker turnaround time and immediate access to laboratory test results for
surveillance purposes, Maine CDC requests that all specimens for arboviral testing be
submitted to HETL, rather than commercial laboratories.
For information on obtaining and
submitting specimens for diagnostic testing call 1-800-821-5821.